High-throughput single cell, organ function measurement and nano hydrogel culture system
High-throughput single cell, organ function measurement and nano hydrogel culture system(excited contraction coupling, nano-fluorescence signal) is a high-throughput measurement unit to easily quantify cell movement. It can evaluate various motion ranges, cell motion trajectories, individual cell strength, hardness and ion concentration from the microscopic level of the cells to the macroscopic level of the tissues, such as measuring the contraction and rapid changes of ion concentration of more than 200 single cells in one hole per hour (Such as calcium transients). The detection of high-throughput cell tension, ion channels, simultaneous measurement of cells and tissues truly realizes the functional detection of a single cardiomyocyte.
There are two models for option.Mcell is manually controlled while Hcell is fully automatic.User can select the models as per their application.
Objects to be measured:
Cancer cells, zebrafish, sperm, colonies, adherent cells (such as acutely isolated adult mouse cells, neonatal mouse cells, adult rat cardiomyocytes, skeletal muscle cells, neuronal stem cells and human stem cells).
Features
1. Obtain accurate cell information
The high throughput single cell, organ function measurement and nano hydrogel 3D culture system provide more accurate analysis data in determining the precise effects of drugs or compounds on contraction, relaxation, or contraction and relaxation. Such as simulating the physiological shape of cardiomyocytes and measuring the rigid environment of organs and tissues. It can measure the speed of cell contraction and relaxation, shortened length (displacement), duration of contraction relaxation, contraction synchronization, contraction propagation, and direction of contraction.
2. Save loads of cell materials.
Treat different holes as different experiments. The high throughput single cell, organ function measurement and nano hydrogel 3D culture system is designed in two models(automatic and manual), and the same cell is tested at different processing or different time points. A single cell is an independent test object. Batch detection of single cell indicators. Different wells can detect the indicators of a single cell under different conditions. The amount of information is huge, saving a lot of consumables and time costs.
3. Research software detects and analyzes cell behavior from the intracellular level to the tissue level.
3.1 It can detect and quantify cell movement at the sub-micron level, enabling researchers to visualize and analyze the fine movement of target cells with target size and time interval.
3.2 Select the tracking area and movement direction through trajectory analysis. The software can quantify the speed or distance of the migration unit. The frequency analysis in the software can analyze the frequency of cell movement.
4. Simultaneous measurement of cell mechanics and ion channels and other indicators. The test subjects are extensive ranging from single cells and tissues to zebrafish models.
5. Automatically identify the unit and select the area and cell to be measured.
The software can automatically identify cells. This is achieved by identifying the target units in the software and then "registering" them in the software. After the setting is completed, the software can also be set to automatically find target units higher than 6000 objects in the image area. The system can search multiple units at the same time to speed up automatic identification.
6. Heart model software function
The heart model has many specialized functions for analyzing the beating movement of myocardial cells. Graphical output includes systolic, diastolic, propagation, and isochron diagrams, which are used to visually detect cardiac cell abnormalities and cell and daughter cell movement. Such as zebrafish heart high-speed fluorescence imaging.
7.Consumable -Nano-Hydrogel
Plexithermo hydrogel can create the most suitable microenvironment for the experiment by adjusting the hardness of the hydrogel. Types of hydrogel available:
Item No. TCM10B :10kPa no fluorescent beads
Item No. TCM30B :30kPa no fluorescent beads
Item No. TCM10M :10kPa + 488fluorescent beads
Item No. TCM30M :30kPa + 488fluorescent beads
Item No.TCM10U :10kPa + 594fluorescent beads
Item No.TCM30U :30kPa + 594fluorescent beads
The main components of the hydrogel include: Acrylamide (Biotech A601032), Bisacrylamide (Biotech A100172,), water.